The University of Lübeck would like to fill the following vacancy at the Institute of Neurogenetics (Head: Prof. Dr. Christine Klein):

Laboratory technician (m/f)

    • Fixed-term contract 24 months, 40h/week
    • Employee status (start date: as soon as possible)

Research area:
Exploring the molecular mechanisms of reduced penetrance in genetic Parkinson’s disease within the framework of a DFG-funded research unit www.protect-move.de)

Your role:
The applicant will be a member of the Mitochondrial Function in Movement Disorders Section (Head: Prof. Dr. Anne Grünewald). He/she will be in charge of cell culture at the Induced Pluripotent Stem Cell (iPSC) Platform (Head: Prof. Dr. Philip Seibler). This includes culturing of patient-derived fibroblasts, generation of iPSC and their differentiation into neurons. Additionally, he/she will perform experiments to assess gene expression and protein profiles as well as cellular function in the above-mentioned model systems. The applicant will also be responsible for general maintenance-related tasks in the laboratory.

Your tasks:
    • Cell culture (cell lines, primary cells, maintenance and neuronal differentiation of iPSCs)
    • RNA/DNA extraction, real-time PCR, sequencing, cloning of plasmids and viral constructs
    • Standard molecular biology (Bradford, ELISA, Western blotting, immunocytochemistry) and cellular
      function (Seahorse technology, colorimetric dyes, enzyme kinetics with spectrophotometry etc.) assays
    • Contribution to general laboratory organisation (ordering, overseeing of stocks, storage, preparation of
      solutions etc.)
    • Autonomous preparation of the research findings for publication in peer-reviewed journals
Your profile:
    • A technical or Bachelor degree in molecular biology, bioengineering, biology or similar
    • Previous experience of working in a cell culture laboratory
    • Excellent organizational skills
    • Good computer skills for standard software (Excel, Word, PowerPoint etc.)
    • Flexible regarding research stays in collaborators’ research laboratories
    • Good working knowledge of English
We offer:
    • Enthusiastic interdisciplinary team of physicians and basic scientists in the area of Parkinson’s disease/neurodegeneration
    • Opportunity to participate in the development of new concepts for the understanding and modulation of Parkinson’s disease
    • An exciting international research environment
Applications (in English or German) should contain the following documents:
    • Cover letter
    • A detailed curriculum vitae with copies of relevant certificates
    • Name and addresses of two referees
    • Autonomous preparation of the research findings for publication in peer-reviewed journals

Please send applications to Prof. Dr. Anne Grünewald at anne.gruenewald@neuro.uni-luebeck.de